Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cloning and Expression
Enzymes
PI-SceI
Supplier Images
PI-SceI
PI-SceI from New England Biolabs
Be the first to write a review!
Citations:
(7)
Supplier Page
Supplier Page from
New England Biolabs for
PI-SceI
Get Pricing
Description
The intein encoding PI-SceI is present in the VMA ATPase gene Saccharomyces cerevisiae (1,5). The gene has been modified for independent expression in E. coli using a T7 RNA polymerase expression system .
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
PI-SceI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0696S
Quantity
250 units
Concentration
1,000 units/ml
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
ATCTATGTCGGGTGC/GGAGAAAGAGGTAATGAAATGGCA
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (7)
(1)
Genome-wide identification of notochord enhancers comprising the regulatory landscape of the brachyury locus in mouse
Development
November 15, 2023
Dennis Schifferl, Manuela Scholze-Wittler, Alba Villaronga Luque, Milena Pustet, Lars Wittler, Jesse V. Veenvliet, Frederic Koch, Bernhard G. Herrmann
random integration of BACs, 5 µg of each construct were linearized using
PI-SceI
(
New England Biolabs
,
R0696S
) and electroporated into 3×106
mESCs
. Approximately 30 h after
electroporation
, selection was initiated,
(2)
Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration
iScience
October 20, 2023
Selena Wei-Zhang, Bohao Cui, Man Xing, Jiaojiao Liu, Yingying Guo, Kai He, Tinghui Bai, Xue Dong, Yi Lei, Wei Zhou,
et al.
and recombinant proteins" ,,
SrfI
,
New England Biolabs
,Cat#
R0629S
PI-SceI
,
New England Biolabs
,Cat#
R0696S
I-CeuI
,
New England Biolabs
,Cat#
R0699S
PacI
,
New England Biolabs
,Cat#
R0547L
ApaI
,
New England Biolabs
,Cat#
R0114S
MfeI-HF
,
New England Biolabs
(3)
A 37 kb region upstream of
brachyury
comprising a notochord enhancer is essential for notochord and tail development
Development
December 1, 2021
Dennis Schifferl, Manuela Scholze-Wittler, Lars Wittler, Jesse V. Veenvliet, Frederic Koch, Bernhard G. Herrmann
integration of the Noto::H2B-mCherry BAC, 5 µg of BAC DNA were linearized using
PI-SceI
(
New England Biolabs
R0696S
) and electroporated into 3×106 wild-type
mESCs
. Approximately 30 h after
electroporation
, selection
(4)
Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering Toolkit
ACS Synthetic Biology
May 15, 2020
Binhui Zhao, Pankaj Chaturvedi, David L. Zimmerman, Andrew S. Belmont
(
New England Biolabs
, Cat. #
R3189S
) and all other BACs with the
PI-SceI
(
New England Biolabs
, Cat. #
R0696S
). Lipofectamine 2000 (
Thermo Fisher Scientific
, Cat. #
11668019
) was used to
transfect
the cells with
(5)
Anti-Depressant Fluoxetine Reveals its Therapeutic Effect Via Astrocytes
EBioMedicine
June 1, 2018
Kinoshita M, Hirayama Y, Fujishita K, Shibata K, Shinozaki Y, Shigetomi E, Takeda A, Le HPN, Hayashi H, Hiasa M,
et al
.
cassette containing tTS and SV40 polyadenylation signal. BAC DNA was linearized by
PI-SceI
(Cat. #
R0696S
,
New England Biolabs
Inc., Massachusetts, U.S.A) enzyme digestion, and injected into
fertilized eggs
(6)
An efficient method of directly cloning chimpanzee adenovirus as a vaccine vector
Nature Protocols
November 1, 2010
Dongming Zhou, Xiangyang Zhou, Ang Bian, Hua Li, Heng Chen, Juliana C Small, Yan Li, Wynetta Giles-Davis, Zhiquan Xiang, Hildegund C J Ertl
no.
R0604S
)
I-CeuI
(
New England Biolabs
, cat. no.
R0699S
)
PI-SceI
(
New England Biolabs
, cat. no.
R0696S
)
SgfI
(
Promega
, cat. no.
R5104
)
Eco47III
(
Promega
, cat. no.
R6731
) Phusion hot start high-fidelity
(7)
Rapid bacterial artificial chromosome modification for large-scale mouse transgenesis
Nature Protocols
October 2, 2010
Shiaoching Gong, Laura Kus, Nathaniel Heintz
endonuclease (
New England Biolabs
, cat. no.
R0180L
)
PI-SceI
endonuclease (
New England Biolabs
, cat. no.
R0696S
) λ-DNA-HindIII Digest (
New England Biolabs
, cat. no.
N3012S
)
Low-range PFG marker
DNA ladder (
New England Biolabs
<<
<
>
>>
Reviews
Be the First to Write a Review!
PI-SceI from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top